本研究对鹅观草属(Roegneria) 13个物种29份材料进行条锈病田间鉴定和抗条锈病基因检测,将筛选的抗病材料与小麦进行人工属间杂交,并对属间杂种F1进行形态学、细胞遗传学及小麦条锈病抗性检测,结果显示:82.76%的材料在田间对条锈病表现中抗以上的抗性,且均含有5个以上的已知抗条锈病基因的等位基因,但仍可能存在条锈病抗性新基因;筛选到的一份高抗条锈病的纤毛鹅观草(Roegneria ciliaris (Trin.) Nevski) ZY11004-R,将其与5个普通小麦(Triticum aestivum L.)品种进行人工属间杂交,通过胚拯救的方式成功获得了纤毛鹅观草-普通小麦CSph2a属间杂种F1;杂种F1体细胞染色体条数为35条(基因组组成为StYABD),花粉母细胞减数分裂I中期染色体多为单价体,形态特征处于两亲本之间,但在生活习性上获得多年生的性状,并表现出高抗条锈病。
In this study, we evaluated the stripe rust resistance type and estimated the published resistance genes in 13 Rogneria species including 29 materials. Afterward, we conducted artificial crosses and investigated the morphology characters, genome constitution, and stripe rust resistance of the intergeneric hybrid F1. The results showed that: 82.76% of the 29 tested materials exhibited stripe rust resistance at adult stage, and they contained more than 5 alleles of stripe rust resistance genes, which might still carry new resistance genes to wheat stripe rust. An intergeneric hybrids R. ciliaris-CSph2a F1 was generated using the screened high stripe rust resistance Roegneria ciliaris (Trin.) Nevski (ZY11004-R) and common wheat mutant CSph2a, and raised based on the embryo rescue technology. F1 hybrid contained 35 chromosomes with StYABD genome constitution and an average of 26.84 monovalents during pollen mother cell metaphase I. Besides, F1 exhibited morphological intermediacy, except the perennial living was inherited form from maternal R. ciliaris with high resistance to wheat stripe rust.