), 姚敏, 任睿, 贾元, 向星汝, 李文, 何昕, 刘忠松, 官春云, 钱论文(), 熊兴华()
), YAO Min, REN Rui, JIA Yuan, XIANG Xing-Ru, LI Wen, HE Xin, LIU Zhong-Song, GUAN Chun-Yun, QIAN Lun-Wen(), XIONG Xing-Hua()
油菜(Brassica napus L.)是中国食用植物油的主要来源, 提高种子含油量是增加菜籽油供应最为有效的方法。本研究利用4个油菜自交系授粉后25 d、35 d、45 d的种子转录组数据分析, 筛选出43个与油脂合成相关基因, 其中33个基因持续上调表达, 10个基因持续下调表达, 主要基因包括BnLEC1、BnABI5、BnOLEO4和BnOBAP1a等。同时, 结合50份半冬性甘蓝型油菜重测序数据, 检测到与含油量显著相关3个SNP、9个SNP分别定位到BnOBAP1a-A10和BnABI5-A05, 其中BnOBAP1a-A10_Hap1对应材料含油量显著高于Hap2, BnABI5-A05_Hap1对应材料含油量显著高于Hap3。此外, 利用WGCNA构建基因共表达网络发现, BnOBAP1a与BnABI5通过3个转录因子LEC1、HMGB3、HTA11间接相连, 形成了潜在调控的分子网络, 影响种子油脂积累。这些结果有利于我们开发单体型功能标记进一步提高油菜籽含油量。
Rapeseed (Brassica napus L.) is the main source of edible vegetable oil in China, and increasing seed oil content is the most effective way to increase the supply of rapeseed oil. In this study, 43 genes related to lipid synthesis were selected by analyzing the seed transcriptome data of 4 rapeseed inbred lines 25, 35, and 45 days after pollination. Among them, 33 genes were continuously up-expressed and 10 genes were continuously down-expressed from 25 to 45 days of seed development. The main genes included BnLEC1, BnABI5, BnOLEO4, and BnOBAP1a. At the same time, combined with the resequencing data of 50 semi-winter Brassica napus, 3 SNPs and 9 SNPs significantly related to oil content were detected to BnOBAP1a-A10 and BnABI5-A05, respectively, and the oil content of BnOBAP1A-A10_Hap1 was significantly higher than Hap2. The oil content of BnABI5-A05_Hap1 was significantly higher than Hap3. In addition, WGCNA was used to construct gene networks, and we found that BnOBAP1a and BnABI5 were indirectly connected through three transcription factors LEC1, HMGB3, and HTA11, which together formed a molecular network involved in the potential regulation of seed oil accumulation. The results of this study provide valuable insights for the development of haplotype functional markers, aiming to further enhance oil content in B. napus.