), 辛旭霞2, 冯智尊2, 曹越2, 郭娟2, Dipak K SANTRA3, 王瑞云2,*(), 陈喜明1,4,*()
2山西农业大学农学院, 中国山西太谷 030801
3内布拉斯加大学林肯分校农艺系小宗粮豆研究与推广中心, 美国内布拉斯加州斯克茨布拉夫 69361
4山西农业大学杂粮种质创新与分子育种山西省重点实验室, 中国山西太原 030000
), XIN Xu-Xia2, FENG Zhi-Zun2, CAO Yue2, GUO Juan2, Dipak K SANTRA3, WANG Rui-Yun2,*(), CHEN Xi-Ming1,4,*()
2College of Agronomy, Shanxi Agricultural University, Taigu 030801, Shanxi, China
3Panhandle Research and Extension Center, Department of Agronomy and Horticulture, University of Nebraska- Lincoln, Scottsbluff 69361, Nebraska, USA
4Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, Shanxi Agricultural University, Taiyuan 030000, Shanxi, China
本研究以500份东北春播区糜子资源为材料, 利用169个SSR标记, 采用UPGMA聚类分组, 进行分层抽样, 构建核心种质, 同时应用ID Analysis 4.0软件构建分子身份证。利用等位基因数(Na)等遗传多样性衡量指标评估核心种质的遗传差异, 并利用PCOA分析核心种质。结果表明, 对169对SSR引物进行筛选, 发现30对多态性好, 利用30对SSR引物构建的糜子核心种质包含190份材料, 占全部种质的38%, 全部种质与核心种质的均检测出91个等位变异, 保留了100%等位基因; 有效等位基因数为2.2977~2.9975和2.2872~3.0173, 平均值分别为2.8198和2.8297; Shannon多样性指数为0.9532~1.0990和0.9535~1.1162, 平均值为1.0645和1.0667; 观测杂合度为0.3434~0.8037和0.3162~0.7849, 平均值为0.5399和0.5359; 期望杂合度为0.5654~0.6672和0.5645~0.6707, 平均值为0.6448和0.6473; Nei’s基因多样性指数为0.5648~0.6664和0.5628~0.6686, 平均值为0.6441和0.6452; 多态性信息含量为0.6657~0.8356和0.6493~0.8340, 平均值为0.7974和0.7944。全部种质与核心种质的分子标记的相关指标进行t检验, 结果无显著性差异, 且PCOA分析表明核心种质与全部种质具有相似的遗传多样性和群体结构, 同时发现8个SSR标记(RYW5、RYW8、RYW16、RYW28、RYW40、RYW53、RYW62和RYW67)可区分190份核心种质, 构建了东北糜子核心种质的分子身份证。
In this study, 500 proso millet resources in Northeast Spring Sowing Region were used as the experimental materials, 169 SSR markers, UPGMA clustering, and stratified sampling were used to construct core germplasm, and ID Analysis 4.0 software was used to construct molecular identity card. The genetic diversity of the core collection was evaluated by genetic diversity metrics such as allele number (Na), and the core collection was analyzed by PCOA. The results showed that 169 pairs of SSR primers were screened, and 30 pairs of SSR primers were found to have good polymorphism. The core collection of proso millet constructed by 30 pairs of SSR primers contained 190 materials, accounting for 38% of all germplasm. Ninety-one alleles were detected in all germplasm and core collection, and 100% alleles were retained. The number of effective alleles was 2.2977-2.9975 and 2.2872-3.0173, with an average of 2.8198 and 2.8297, respectively. The Shannon diversity index was 0.9532-1.0990 and 0.9535-1.1162, with an average of 1.0645 and 1.0667. The observed heterozygosity was 0.3434-0.8037 and 0.3162-0.7849, with an average of 0.5399 and 0.5359. The expected heterozygosity was 0.5654-0.6672 and 0.5645-0.6707, with an average of 0.6448 and 0.6473. Nei’s gene diversity index was 0.5648-0.6664 and 0.5628-0.6686, with an average of 0.6441 and 0.6452. The polymorphism information content was 0.6657-0.8356 and 0.6493-0.8340, with an average of 0.7974 and 0.7944. The results of t-test showed that there was no significant difference in the related indexes of molecular markers between all germplasm and core germplasm, and PCOA analysis showed that the core germplasm and all germplasm had similar genetic diversity and population structure. At the same time, 8 SSR markers (RYW5, RYW8, RYW16, RYW28, RYW40, RYW53, RYW62, and RYW67) were found to distinguish 190 core germplasms, and the molecular identity card of the core germplasms of Northeast proso millet was constructed, which providing a scientific basis for the efficient utilization and rapid traceability of proso millet germplasm.